WGS data-based consensus sequence recovery and visualization using GeneiousPrime®: A technical approach with Ogye Chicken data

Thisarani Kalhari  Ediriweera1   Jun Heon  Lee1,*   

1Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea

Abstract

Whole Genome Sequencing (WGS) provides high throughput sequencing data that reveals the true nature of the complete genome of an individual. The present study has used Ogye chicken WGS data to technically adduce the consensus sequence recovery and visualization of their assembly. Accordingly, they were mapped to NCBI accession: NC_052547.1 (of GRCg7b) using the Geneious mapper of Geneious Prime® software package. The mapping procedure was described step by step with the settings used, and visualizations were illustrated. This technique can effectively be applied in the Geneious Prime® platform to consensus sequence recovery coupled with appealing and elaborative visualizations.

Figures & Tables

Fig. 1. Raw sequence reads imported to the Geneious Prime software (Ogye_F : forward sequence file is visualized)