Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Nuri Choi1, Minjun Kim2, Yusam Kim1, Do Hyun Kim3, Hak Kyo Lee3, Myung Hum Park1, Bong Hwan Choi4, Dongwon Seo1*
1R&D Center, TNT Research Ltd., Sejong 30141, Korea
2Department of Animal Science, Chungnam National University, Daejeon 34134, Korea
3Department of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Korea
4Animal Genetic Resource Center, National Institute of Animal Science, Hamyang, Korea
Correspondence to Dongwon Seo, E-mail: dwseo@tntresearch.co.kr
Volume 8, Number 4, Pages 227-235, December 2024.
Journal of Animal Breeding and Genomics 2024, 8(4), 227-235. https://doi.org/10.12972/jabng.20240415
Received on 17 December, 2024, Revised on 24 December, 2024, Accepted on 26 December, 2024, Published on 31 December, 2024.
Copyright © 2024 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
The identity and parentage verification tests for Hanwoo play a crucial role not only in preventing fraudulent distribution in the market but also in generating greater profits in calf transactions by verifying the parentage of superior bloodlines. Therefore, this study secured the genotypes of 946 Hanwoo cattle using the GeneTrackTM Ver1 Genotyping Kit (GT1), based on the dinucleotide marker combination recommended by ISAG, and the GeneTrackTM Ver2 Genotyping Kit (GT2), based on a combination of tri- to hexanucleotide markers, to compare the results of paternity matches and mismatches for the same animals in the Jeonbuk region. Among the 17 individuals identified as mismatches using the Hanwoo Chip SNP genotypes, 3 animals showed the same mismatch results with SNPs using the GT1 marker combination, while 14 animals were confirmed as mismatches. In the GT2 marker combination, 16 animals showed mismatches, and 1 animal was confirmed as a mismatch. These findings highlight the necessity of verifying marker genotypes and determination errors more accurately through additional samples and the configuration of Trio samples.
Hanwoo, Microsatellite markers (MS), parentage test, SNP, identity
본 연구는 농협한우개량사업소의 KPN에 대한 GeneTrackTM Ver2 Genotyping Kit 유전자형 분석에 샘플을 협조해주신 내용에 감사드리고, 본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원의 기술사업화지원사업(RS-2022-IP122050)의 지원을 받아 연구되었습니다. 연구비 지원에 감사드립니다.
No potential conflict of interest relevant to this article is reported.
Brenig B and Schütz E. 2016. Recent development of allele frequencies and exclusion probabilities of microsatellites used for parentage control in the German Holstein Friesian cattle population. BMC Genetics. 17:18.
[DOI][PubMed][PMC]
Chambers GK and MacAvoy ES. 2000. Microsatellites: consensus and controversy. Comparative Biochemistry and Physiology Part B: Biochemistry Molecular Biology. 126:455-476.
[DOI][PubMed]
Chandra D, Mishra VC, Raina A and Raina V. 2022. Mutation rate evaluation at 21 autosomal STR loci: Paternity testing experience. Legal Medicine. 58:102080.
[DOI][PubMed]
Fernández ME, Goszczynski DE, Lirón JP, Villegas-Castagnasso EE, Carino MH, Ripoli MV, Rogberg-Muñoz A, Posik DM, Peral-García P and Giovambattista G. 2013. Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd. Genetics and Molecular Biology 36:185-191.
[DOI][PubMed][PMC]
Herráez DL, Schäfer H, Mosner J, Fries HR and Wink M. 2005. Comparison of Microsatellite and Single Nucleotide Polymorphism Markers for the Genetic Analysis of a Galloway Cattle Population. journal Zeitschrift für Naturforschung C. 60:637-643
[DOI][PubMed]
Huang QY, Xu FH, Shen H, Deng HY, Liu YJ, Liu YZ, Li JL, Recker RR and Deng HW. 2002. Mutation Patterns at Dinucleotide Microsatellite Loci in Humans. The American Journal of Human Genetics. 70:625-634.
[DOI][PubMed][PMC]
Jin S, Won JI, Kim HJ, Park B, Kim SW, Kim UH, Kang SS, Lee HJ, Moon SJ, Park MS, Sim YT, Jang SS and Kim NY. 2024. Polymorphism analysis of tri- and tetranucleotide repeat microsatellite markers in Hanwoo cattle. Journal of Animal Science and Technology. 66:717-725.
[DOI][PubMed][PMC]
Jin S, Won JI, Park B, Kim SW, Kim UH, Kang SS, Lee HJ, Moon SJ, Park MS, Lim HT, Kim EH, Kang HC, Jang SS and Kim NY. 2023. Genetic diversity analysis of the line-breeding Hanwoo population using 11 microsatellite markers. Korean Journal of Agricultural Science. 50:363 ~ 372-363 ~ 372.
[DOI]
Kim HR, Lee JW, Go MJ, Park JE, Kim MJ, Baek YC, Park SH, Lim DJ, Lee SD and Choi BH. 2020. Development of a New Microsatellite Markers for Individual Identification and Paternity Evaluation in Hanwoo. Journal of Agriculture and Life Science. 54:75-83.
[DOI]
Sun DW, Park MS, Choi KM, Kim HK, Kang HC, Lee JG, Lee SS, Moon SC and Lim HT. 2021. Effects Through Parental Identification of Korean Cattle (Hanwoo). Journal of Agriculture and Life Science. 55:75-81.
[DOI]
Lim HT, Min HS, Moon WG, Lee JB, Kim JH, Cho IC, Lee HK, Lee YW, Lee JG and Jeon JT. 2005. Analysis and Selection of Microsatellites Markers for Individual Traceability System in Hanwoo. Journal Animal Science and Technology. 47:491-500.
[DOI]
Schütz E and Brenig B. 2015. Analytical and statistical consideration on the use of the ISAG-ICAR-SNP bovine panel for parentage control, using the Illumina BeadChip technology: example on the German Holstein population. Genetics and Selection Evolution. 47:3.
[DOI][PubMed][PMC]
Sim YT, Na JG and Lee CS. 2013. Forensic Characterization of Four New Bovine Tri-nucleotide Microsatellite Markers in Korean Cattle (Hanwoo). Journal of Animal Science and Technology. 55:87-93.
[DOI]
Yoon DH, Kong HS, Oh JD, Lee JH, Cho BW, Kim JD, Jeon KJ, Jo C, Jeon GJ and Lee H. 2005. Establishment of an Individual Identification System Based on Microsatellite Polymorphisms in Korean Cattle (Hanwoo). Asian-Australasian Journal of Animal Science 18:762-766.
[DOI]