Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Yuju Lee1†, Dain Lee2†, Julan Kim2, Tae-Hee Kim1, Chaehyeon Lim2, Hyejin Kim2, Jong-Won Park2, Yoonji Chung3, Yeongkuk Kim4, Min Sung Kim5, Seung Hwan Lee3, Woo-jai Lee5, Hyun-Chul Kim2*, Hee-Bok Park1
1Department of Animal Resources Science, Kongju National University, Yesan, 32439, Korea
2Genetics and Breeding Research Center, National Institute of Fisheries Science, Geoje 53334, Korea
3Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea
4Quantomic, Deajeon 34167, Korea
5BLUGEN, Busan 48071, Korea
Correspondence to Hee-Bok Park, E-mail: heebokpark@kongju.ac.kr
†These authors contributed equally to this work.
Volume 8, Number 4, Pages 107-116, December 2024.
Journal of Animal Breeding and Genomics 2024, 8(4), 107-116. https://doi.org/10.12972/jabng.20240401
Received on 28 October, 2024, Revised on 09 December, 2024, Accepted on 09 December, 2024, Published on 31 December, 2024.
Copyright © 2024 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
The olive flounder (Paralichthys olivaceus) is one of the most economically important fish species in South Korea’s aquaculture industry. However, Edwardsiellosis, caused by Edwardsiella tarda (E. tarda), poses a significant threat to this industry, resulting in high mortality rates and economic losses. The aim of this study is to identify genetic loci associated with Edwardsiellosis resistance in olive flounder using a genome-wide association study (GWAS). A challenge experiment was conducted, infecting 2,939 flounders with E. tarda and recording survival traits. Genotyping was performed using a 60K SNP array, followed by statistical genetic analysis using SPACox software, which identified four significant SNPs on chromosomes 10, 13, 18, and 21. These SNPs were located in or near positional candidate genes for inflammation and immune responses, such as STXBP5L, MEF2D, and HOMER1, indicating their potential involvement in bacterial defense mechanisms. Biological pathway analysis revealed several significant pathways, including Apelin signaling, ECM-receptor interaction, Calcium signaling, and PI3K-Akt signaling, which are known to regulate immune cell activity and inflammation. These findings provide important insights into the genetic mechanisms of disease resistance in olive f lounder and offer valuable resources for future selective breeding programs aimed at enhancing Edwardsiellosis resistance.
Disease, Edwardsiellosis, Genome-wide association study, Olive flounder, SNP
이 논문은 국립수산과학원(수산과학연구사업 R2024032)의 지원으로 수행되었습니다.
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