Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Jeong-Woong Park1, Jae-Young Choi2, Kyoung Hwan Kim3, Sujung Kim4, Jae-Rung So5, Byeong-Woo Kim6, Byung-Wook Cho6*
1Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), 241, Cheomdangwahag-ro, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea
2Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, 63242, Korea
3Jeju Racing Resources Management Team, Jeju Racecourse Division, Korea Racing Authority, Jeju, 63066, Republic of Korea
4The Animal Molecular Genetics and Breeding Center, Jeonbuk National University, 54896, Republic of Korea
5Department of Agricultural Convergence Technology, Jeonbuk National University, 54896, Republic of Korea
6Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea
Correspondence to Byung-Wook Cho, E-mail: bwcho@pusan.ac.kr
Volume 7, Number 4, Pages 133-142, December 2023.
Journal of Animal Breeding and Genomics 2023, 7(4), 133-142. https://doi.org/10.12972/jabng.20230015
Received on 16 October, 2023, Revised on 15 December, 2023, Accepted on 15 December, 2023, Published on 31 December, 2023.
Copyright © 2023 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
Since horse industry get bigger with horse racing and horse riding, athletic performance become most important trait on horses, but the molecular analysis and regulatory pathway studies remain insufficient for evaluation and prediction of horse athletic abilities. In our previous study, we conducted RNA-sequencing analysis with muscle and blood samples by exercise in Thoroughbreds. Through RNA-sequencing, we identified Pleckstrin Homology and RhoGEF Domain Containing G1 (PLEKHG1) gene expressed differentially by alternative spliced isoforms in skeletal muscle during exercise. In this study, we conducted reverse transcriptase polymerase chain reaction (RT-PCR) to validate two isoforms of equine PLEKHG1 transcripts (PLEKHG1a, PLEKHG1b), and cloned the transcripts to confirm the sequences. Additionally, we validated expression pattern of PLEKHG1a (long form of transcript) and PLEKHG1b (short form of transcript) in horse tissue by quantitative RT-PCR (qRT-PCR). Prediction of protein structure of these isoforms revealed two putative phosphorylation sites at the amino acid sequences encoded in exon 7, which is deleted in PLEKHG1b. Expression pattern of PLEKHG1a and PLEKHG1b shows cross expressed pattern as RNA-sequencing data. PLEKHG1a increased after exercise whereas PLEKHG1b decreased after exercise. Collectively, it is assumed that the expression patterns of PLEKHG1a and PLEKHG1b transcripts would be involved in regulation of myogenic differentiation and myogenic proliferation through the Ras signaling pathway. Further study should be necessary to uncover biological function(s) and significance of the alternative splicing isoforms in equine skeletal muscle.
Horse, Pleckstrin Homology and RhoGEF Domain Containing G1, Alternative Splicing, Athletic Performance, Muscle, RNA-Sequence
This work was supported by a 2-Year Research Grant of Pusan National University.
We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.
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