Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Indexed in KCI
OPEN ACCESS, PEER REVIEWED
pISSN 1226-5543
eISSN 2586-4297
Research Article

Molecular evidence for occurrence of endangered long-tailed goral in Seoul metropolitan city, South Korea

1National Park Institute for Wildlife Conservation, Korea National Park Service, Yeongju, 36015, Korea
2Korea Zoonosis Research Institute(KoZRI), Chonbuk National University, Iksan, 54531, Korea
3Department of Culture & Documentary, Seoul Broadcasting System, Seoul, 03916, Korea

Correspondence to Sang-Hyun Han, E-mail: hansh04@knps.or.kr

Volume 7, Number 3, Pages 85-93, September 2023.
Journal of Animal Breeding and Genomics 2023, 7(3), 85-93. https://doi.org/10.12972/jabng.20230010
Received on 30 August, 2023, Revised on 18 September, 2023, Accepted on 22 September, 2023, Published on 30 September, 2023.
Copyright © 2023 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).

ABSTRACT

The study examined the habitation and the number of long-tailed gorals by molecular identification, sexing discrimination, and identity tests on feces and hair samples estimated to be endangered long-tailed goral in Mt. Yongmasan, Mt. Achasan and Mt. Inwangsan near Seoul metropolitan city. Mitochondrial cytochrome oxidase I (COI) gene sequences showed that the DNA samples from the three mountains were those of long-tailed goral (Naemorhedus caudatus). The molecular sexing results showed that there were a female and a male in Mt. Yongmasan, a male in Mt. Achasan, but no informative results for Mt. Inwangsan. The identity test results indicated that the males of Mt. Yongmasan and Mt. Achasan were the same male, estimating the shift of this male from Mt. Yongmasan to Mt. Achasan. In addition, the goral of Mt. Inwangsan had a different COI sequence from those of Mt. Yongmasan and Mt. Achasan estimated to be this male with a different maternal lineage. Our present findings provide molecular evidence that at least three gorals have been inhabiting or continuously visiting the mountainous areas of Seoul metropolitan city, suggesting that a management system may be necessary for protecting this natural habitat of endangered long-tailed goral.

KEYWORDS

endangered species, genetic identification, long-tailed goral, Naemorhedus caudatus, Seoul metropolitan city

ACKNOWLEDGEMENTS

본 논문은 국립공원공단 국립공원야생생물보전원의 “멸종위기종 증식‧복원 사업”에서 수행된 결과의 일부이며, 이에 감사드립니다.

REFERENCES

An J, Choi SK, Sommer J, Louis E, Brenneman R, Zemanova B, Hajkova P, Park G, Min MS, Kim KS and Lee H. 2010. A core set of microsatellite markers for conservation genetics studies of Korean goral (Naemorhedus caudatus) and its cross-species amplification in Caprinae species. J. Vet. Sci. 11:351-353.
[DOI][PubMed][PMC]

An J, Min MS, Sommer J, Louis E, Brenneman R, Kwon SW, Shin NS and Lee H. 2005. Isolation and characterization of 15 microsatellite loci in the Korean goral (Nemorhaedus caudatus). Mol. Ecol. Notes 5:421-423.
[DOI]

Ancona S, Dénes FV, Krüger O, Székely T and Beissinger SR. 2017. Estimating adult sex ratios in nature. Philos. Trans. R. Soc. Lond. B Biol. Sci. 372:20160313.
[DOI][PubMed][PMC]

Bhandari S, Morley C, Aryal A and Shrestha UB. 2019. The diet of the striped hyena in Nepals’s lowland regions. Ecol. Evol. 10:7953-7962.
[DOI][PubMed][PMC]

Blundell GM, Ben-David M, Groves P, Bowyer RT and Geffen E. 2002. Characteristics of sex-biased dispersal and gene flow in coastal river otters: implications for natural recolonization of extirpated populations. Mol. Ecol. 11(3):289-303.
[DOI][PubMed]

Bohmann K, Evans A, Gilbert MTP, Carvalho GR, Creer S, Knapp M, Yu DW and de Bruyn M. 2014. Environmental DNA for wildlife biology and biodiversity monitoring. Trends Ecol. Evol. 29:358-367.
[DOI][PubMed]

Bowler DE and Benton TG. 2005. Causes and consequences of animal dispersal strategies: relating individual behaviour to spatial dynamics. Biol. Rev. 80:205-225.
[DOI][PubMed]

Cho CU. 2013. Systematic study on the long-tailed-goral (Naemorhedus caudatus), with ecology and conservation plan. Ph. D. Dissertation, Chungbuk Nat’l Univ., pp. 72-119. (in Korean with English abstract)

Cho CU, Kim KC, Kim KY, Lee YH, Son JI, Jung SJ, Song DJ and Jung DH. 2016. Analysis of home range change of female goral (Naemorhedus caudatus) during the period if birth and management plan for their protection in the natural habitat in Korea. Korean J. Environ. Ecol. 30(5):857864.
[DOI]

Cho CU, Kim KY, Kim KC, Kim HM, An JY, Lee BK and Park JG. 2015. Home range analysis of a apir of gorals (Naemorhedus caudatus) using GPS collar according to the elevation change, in the North Gyeongbuk Province (Uljin) of Korea. Journal of the Korean Association of Geographic Information Studies 18(1):135-144.
[DOI]

Clobert J, Galliard L, Cote J, Meylan S and Massot M. 2009. Informed dispersal, heterogeneity in animal dispersal syndromes and the dynamics of spatially structured populations. Ecol. Lett. 12:197-209.
[DOI][PubMed]

Dahle B, Støen O and Swenson JE. 2006. Factors influencing home-range size in subadult brown bears. J. Mammal 87(5):859-865.
[DOI]

De Villiers FA and Measey J. 2017. Overland movement in African clawed frogs (Xenopus laevis): empirical dispersal data from within their native range. Peer J. 5:e4039.
[DOI][PubMed][PMC]

Fernando P, Vidya TNC, Rajapakse C, Dangolla A and Melnick DJ. 2003. Reliable noninvasive genotyping: fantasy or reality? J. Hered. 94:115-123.
[DOI][PubMed]

Greenwood PJ. 1980. Mating systems, philopatry and dispersal in birds and mammals. Anim. Behav. 28:1140-1162.
[DOI]

Hammond RL, Handley LJL, Winney BJ, Bruford MW and Perrin N. 2006. Genetic evidence for female-biased dispersal and gene flow in a polygynous primate. Proc. R. Soc. B. 273:479-484.
[DOI][PubMed][PMC]

Handley LJL and Perrin N. 2007. Advances in our understanding of mammalian sex‐biased dispersal. Mol. Ecol. 16(8):1559-1578.
[DOI][PubMed]

Jo YS, Baccus JT and Koprowski JL. 2018. Mammals of Korea. National Institute of Biological Resources, Incheon, pp. 309-310.

Johnson ML and Gaines MS. 1990. Evolution of dispersal: theoretical models and empirical tests using birds and mammals. Annu. Rev. Ecol. Syst. 21:449-480.
[DOI]

Kerth G, Mayer F and Petit E. 2002. Extreme sex‐biased dispersal in the communally breeding, nonmigratory Bechstein’s bat (Myotis bechsteinii). Mol. Ecol. 11(8): 1491-1498.
[DOI][PubMed]

King RA, Read DS, Traugott M and Symondson WO. 2008. Molecular analysis of predation: a review of best practice for DNA-based approaches. Mol. Ecol. 17(4):947-963.
[DOI][PubMed]

Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ and Higgins DG. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23:2947-2948.
[DOI][PubMed]

Le Galliard JF, Fitze PS, Ferriere R and Clobert J. 2005. Sex ratio bias, male aggression, and population collapse in lizards. Proc. Natl Acad. Sci. USA 102:18231-18236.
[DOI][PubMed][PMC]

McIellan B and Hovey FW. 2001. Natal dispersal of grizzly bears. Canad. J. Zool. 79(5):838-844.
[DOI]

Mucci N, Mengoni C and Rnadi E. 2014. Wildlife DNA forensic against crime: Resolution of a case tortoise theft. Forensic Sci. Int. Genet. 8:200202.
[DOI][PubMed]

Munshi-South J. 2008. Female-biased dispersal and gene flow in a behaviorally monogamous mammal, the large treeshew (Tupaia tana). PLoS One 3:e3228.
[DOI][PubMed][PMC]

Myslenkov AI and Voloshina V. 1998. Sexual behavior of Amur goral. Proceeding of 2nd World Congress of Mt. Ungulates, pp. 75-80.

Ogden R and Linacre A. 2015. Wildlife forensic science: A review of genetic geographic origin assignment. Forensic Sci. Int. Genet. 18:152-159.
[DOI][PubMed]

Opel KL, Chung D and McCord BR. 2010. A study of PCR inhibition mechanisms using real time PCR. J. Forensic Sci. 55(1):25-33.
[DOI][PubMed]

Pusey AE and Packer C. 1987. The evolution of sex-biased dispersal in lions. Behaviour 101(4):275-310.
[DOI]

Peeters B, Moullec ML, Raeymaekers JAM, Marquez JF, Roed KH, Pedersen AO, Veiberg V, Loe LE and Hansen BB. 2019. Sea ice loss increases genetic isolation in a high Arctic ungulate metapopulation. Glob. Chang. Biol. 26:2028-2041.
[DOI][PubMed]

Ramon-Laca A, Soriano L, Gleeson D and Godoy JA. 2015. A simple and effective method for obtaining mammal DNA from faeces. Wildl. Biol. 21:195-203.
[DOI]

Roy J, Yannic G, Cote S and Bernatchez L. 2012. Negative density-dependent dispersal in the American black bear (Ursus americanus) revealed by noninvasive sampling and genotyping. Ecol. Evol. 2:525-537.
[DOI][PubMed][PMC]

Robbins A, Stoinski T, Fawcett K and Robbins M. 2009. Socioecological influence on the dispersal of female mountain gorillas-evidence of a second folivore paradox. Behav. Ecol. Sociobiol. 63:477-489.
[DOI]

Staats M, Arulandhu AJ, Gravendeel B, Holst-Jensen A, Scholtens I, Peelen T, Prins TW and Kok E. 2016. Advances in DNA metabarcoding for food and wildlife forensic species identification. Anal. Bioanal. Chem. 408:4615-4630.
[DOI][PubMed][PMC]

Steffen P, Eggen A, Dietz AB, Womack JE, Stranzinger G and Fries R. 1993. Isolation and mapping of polymorphic microsatellites in cattle. Anim. Genet. 24:121-124.
[DOI][PubMed]

Taberlet P, Griffin S, Goossens B, Questieau S, Manceau V, Escaravage N, Waits LP and Bouvet J. 1996. Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acid Res. 24:3189-3194.
[DOI][PubMed][PMC]

Tamura K, Stecher G, Peterson D, Filipski A and Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729.
[DOI][PubMed][PMC]

Toldo SS, Fries R, Steffen P, Neiberg HL, Barendse W, Womack JE, Hetzel DJS and Stranzinger G. 1993. Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes. Mamm. Genome 4:720-727.
[DOI][PubMed]

Veran S and Beissinger SR. 2009. Demographic origins of skewed operational and adult sex ratios: perturbation analyses of two-sex models. Ecol. Lett. 12:129-143.
[DOI][PubMed]

Weiß BM, Kulik L, Ruiz-Lambides AV and Widdig A. 2016. Individual dispersal decisions affect fitness via maternal rank effects in male rhesus macaques. Sci. Rep. 6:32212.
[DOI][PubMed][PMC]

Wilson DE and Mittermeier RA. 2011. Handbook of the Mammals of the World. Vol. 2. Hoofed Mammals. Lynx Edicions, Barcelona, pp. 745.

Won PH. 1967. The Illustrated Encyclopedia of Fauna and Flora of Korea. Vol. 7. Ministry of Education, pp. 59-65.

Yang BK. 2002. Systematic, ecology and current population status of the goral, Naemorhedus caudatus, in Korea. Ph. D. Dissertation, Chungbuk Nat’l Univ., pp. 29-58. (in Korean with English abstract)

Yoon MH, Han SH, Oh HS and Kim JG. 2004. The Mammals of Korea. Dongbangmedia, Seoul, pp. 272-273.

Section