Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Chanwoo Lee1, Sunghak Kim3*, Yoonseok Lee1,2*
1School of Biotechnology, Hankyong National University, Gyeonggi-do, Anseong, 17579, Republic of Korea
2Center for Genetic Information, Hankyong National University, Gyeonggi-do, Anseong, 17579, Republic of Korea
3Department of Animal Science, Chonnam National University, Gwangju 61186, Republic of Korea
Correspondence to Yoonseok Lee, E-mail: yoonseok95@hknu.ac.kr; Sunghak Kim, E-mail: sunghakkim@jnu.ac.kr
Volume 6, Number 3, Pages 95-97, September 2022.
Journal of Animal Breeding and Genomics 2022, 6(3), 95-97. https://doi.org/10.12972/jabng.20220010
Received on May 13, 2022, Revised on July 16, 2022, Accepted on August 16, 2021, Published on September 30, 2022.
Copyright © 2022 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
miRNAs are a class of endogenous small non-coding RNAs, approximately 22 nucleotides in length, which play a role in post transcriptional regulation by target mRNA degradation or inhibiting their translation. glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) that catalyze the initial and committed step in glycerolipid biosynthesis has long length of 3´untranslated region (3´UTR) could be found a number of SNPs and affected by miRNA. Therefore, in this study, we aimed to investigate whether bta-miR-2468 binds to the 3´UTR of GPAM, including the g. 55930 C>T SNP, and whether each allele of this SNP affects the binding affinity between the target miRNA and the 3´UTR. For the dual luciferase assay, pmirGLO-55930 C and pmirGLO-55930 T as plasmid DNA includes g. 55930 C>T react bta-miR-2468, identified protein expression with firefly luciferase activity and renilla luciferase activity. As a results, validate the results reported by in silico assay that reported when either g. 55930 C or g. 55930 T were present in the seed region of bta-miR-2468, there was an evident reduction, on the contrary, co-transfection of both the g.55930 SNP allele and bta-miR-2468 did not result in significantly different luciferase activities in HEK293T cell. In this study, we demonstrated the importance of post-transcriptional regulation of GPAM gene expression by bta-miR-2468, Considering the effect of haplotype (g. 54853 A>G – g. 55441 A>G – g. 55930 C>T), the effect of the g.55930 T>C SNP should be evaluated in future studies using pmirGLO vectors, including haplotypes.
bta-miR-2468, GPAM 3´UTR, g. 55930 C>T, Functional SNPs, Korean Native Cattle
This work was supported by a research grant from Hankyong National University in 2020.
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