Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Sola-Ojo, F.E1*., Afolabi-Balogun, N.B2., Adeniyi C.A3., Adeyemi, K.D1., Ayorinde, K.L1., Alli, O.I1., Oni, O.A2., Okeke, C.U4., Momoh E.O1., Adewara, J1., and Abdulkareem, I1.
1Quantitative, Molecular and Functional Genomics Unit, Department of Animal Production, University of Ilorin, Ilorin, Kwara State, Nigeria.
2Molecular Biology and Genetic Diversity Research Laboratory, Biochemistry Unit, Department of Chemical Sciences, and Fountain University Osogbo, Osun State, Nigeria.
3State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
4Department of Prosthetics and Orthotics, School of Health Technology Owerri, Imo State, Nigeria.
Correspondence to Sola-Ojo, F.E, E-mail: mofesola1@yahoo.com
Volume 5, Number 2, Pages 59-70, June 2021.
Journal of Animal Breeding and Genomics 2021, 5(2), 59-70. https://doi.org/10.12972/jabng.20210005
Received on 02 April, 2021, Revised on 27 June, 2021, Accepted on 28 June, 2021, Published on 30 June, 2021.
Copyright © 2021 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
Sexing of birds at early age is very important for efficient selection and breeding; while characterization and taxonomic identification is relevant in conservation of birds’ genetic resources. This study used the genomic DNA of ten (10) guinea fowl keets to determine their sex using agarose gel electrophoresis and sequencing with chromo helicase DNA (CHD) binding genes, they were also characterize taxonomically using 12S rRNA mitochondria genes. The results of this study shows a double band (ZW) for females and a single band (W) for males under Agarose gel electrophoresis view, the Guinea fowl keets sequenced showed some deletions and were closer to Gallus_CHD12 in the phylogenetic tree. The Taxonomic classification result shows that the sequenced guineafowl keets were most related to the Numida meleagris 12S mitochondrial ribosomal RNA. This study corroborate the fact sex of guineafowl keet can be easily identified at genomic DNA level and they can be characterized taxonomically using the 12SrRNA mitochondrial genes.
We are grateful to Dr. Sola-Ojo, F.E Project students (2019/2020 sets) who assisted in sampling and also the management and staff of Fair and Firm Farm Limited, Ilorin, Kwara State, Nigeria for the provision of housing facilities for the keets.
The authors declare no competing interests.