Journal of Animal Breeding and Genomics (J Anim Breed Genom)
ALsaedi Raad Raheem Tolee1,*, Epishko Olga2, Cheburanova Ekaterina2
1Yanka Kupala state university of Grodno. Grodno, Belarus.
2Grodno State Agrarian university. Grodno, Belarus.
Correspondence to ALsaedi Raad Raheem Tolee
Volume 3, Number 4, Pages 107-112, December 2019.
Journal of Animal Breeding and Genomics 2019, 3(4), 107-112. https://doi.org/10.12972/jabng.20190011
Received on 15 October, 2019, Revised on 30 December, 2019, Accepted on 31 December, 2019, Published on December 31, 2019.
Copyright © 2019 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
The calpastatin gene (CAST) is an important marker associated with meat quality in sheep. The aim of our research is to develop methods that allows studying and identifying allelic variants of the CAST gene in sheep at the molecular genetic level, where polymorphism of the CAST gene in sheep was studied using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP analysis). In this study, calpastatin (CAST) gene polimorphism was investigated in three sheep breeds in Belarus and Iraq using PCR-RFLP method. The frequencies of M and N alleles of CAST gene (n=150) were determined as 0,864-0,136. According to chi square test, the population were consistent with Hardy-Weinberg equilibrium were population shows no significant deviation from Hardy-Weinberg equilibrium for the CAST gene.
Polymorphism, CAST gene, Sheep, meat quality.
The authors thank the Agriculture research center from the regions of Grodno-Belarus, Minsk-Belarus, Baghdad-Iraq, respectively.