Journal of Animal Breeding and Genomics (J Anim Breed Genom)
Dyah Maharani1*, Dwi Nur Happy Hariyono1, Sunghyun Cho2, Prabuddha Manjula2, Dongwon Seo2, Nuri Choi2, Jafendi Hasoloan Purba Sidadolog1 and Jun-Heon Lee2
1Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
2Division of Animal and Dairy Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, South Korea
Correspondence to Dyah Maharani, E-mail: d.maharani@ugm.ac.id
Volume 1, Number 2, Pages 136-142, December 2017.
Journal of Animal Breeding and Genomics 2017, 1(2), 136-142. https://doi.org/10.12972/jabng.20170013
Received on 7 December, 2017, Accepted on 26 December, 2017, Published on December 31, 2017.
Copyright © 2017 Korean Society of Animal Breeding and Genetics.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0).
To date, a number of local duck breeds has been widely raised across Java island of Indonesia, namely Mojosari, Magelang, Rambon, and Turi. In order to evaluate the genetic diversity among them, 22 microsatellite markers were used to genotype of 120 individuals (30 individuals per population). Such indicators, including number of alleles (Na), observed heterozigosity (Ho), expected heterozigosity (He), polymorphism information content (PIC), and Wright’s F-statistics (FIT, FIS, FST) were analyzed. A total of 139 alleles were detected in all loci, ranging from 3 (AMU123, CAUD128, CAUD009) to 21 (CAUD048). The mean value of Ho, He, and PIC were 0.465, 0.580, 0.524, respectively, indicating relatively medium genetic diversity among populations. Only 8 loci were not in accordance to Hardy Weinberg equilibrium (P < 0.01). Among the four populations, the global heterozigosity deficit (FIT) was 0,197, global inbreeding of individuals within population (FIS) was 0.112, and population differentiation index (FST) was 0.093. In conclusion, relatively medium genetic diversity and differentiation among populations were successfully estimated using 22 microsatellite markers. The considerable genetic diversity among populations will allow us for future breeding improvement, as well as conservation strategies.
Genetic diversity, Mirosatellite markers, Indonesian local ducks
This work represented an international research collaboration between Universitas Gadjah Mada, Yogyakarta, Indonesia and Chungnam National University, Daejeon, South Korea, supported by the Indonesian Directorate General of Higher Education (DIKTI) with contract No. 2262/UN1-P.III/DIT-LIT/LT/2017. We also thank to the Agricultural and Livestock Bureau of Cirebon, Magelang, Ogan Ilir, Pesisir Selatan, Tanah Datar, and Livestock Breeding Center (BPTU-HPT) Pelaihari for assisting in the collection of blood samples from breeding flocks.