Identification and selection of genetic disorders are very important for breeding strategy and improvement process of livestock. Therefore, selection against the disease-related causative genes and variants is most ideal breeding method to prevent inheritable genetic disorders for future generations. In case of Hanwoo breeding scheme, the standardized identification method for genetic disorders has not yet been established. Therefore, it is necessary to screening investigate basic study for genetic disorders in Hanwoo. The objective of this study was to investigate mutations of four candidate genes, such as Haemophilia A, Chediak-Higashi syndrome (CHS), Spherocytosis, and Xanthinuria Type II to determine the presence of genetic disorders in Hanwoo. A total of 376 Hanwoo samples were screened by PCR-RFLP and KASP assay genotyping method. As the results, fortunately, mutations causing genetic disorders were not identified in the four candidate genes. However, further studies will be needed to investigate more samples and other disease related genes should be monitored for controlling genetic diseases in Hanwoo.